The Basic Principles Of attune flow cytometer
The Basic Principles Of attune flow cytometer
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Information-enriched characterization of mobile populations. Morphological info from pictures can increase to the richness of flow cytometry data. Such as, the figure shows an usually typical apoptosis assay applying Annexin V and PI, incorporating cell imaging to characterize cells in Every populace to reveal morphologically distinctive options. These insights could not are already gained from multiplex staining by itself.
Regardless of whether you’re a rookie or an authority, investigate our resources to advance your investigation and expertise in flow cytometry.
(B) Uncooked 264.seven macrophages are frequently annotated inaccurately with regard to particle count due to their complexity and noncircular borders. The quantity of singlets available for analysis increases as soon as the consumer trains and employs a more precise product.
Learn new insights into your experimental info by visualizing populations working with blended fluorescence and morphology facts. Our software program interface wizards empower downsampling, concatenation (merged documents) and dimensionality applications which have been created in the software for an intuitive, streamlined knowledge Examination knowledge.
"An image is just not really truly worth a thousand dots, but it surely genuinely really helps to see visuals. It is excellent to discover photos that correlate with staining. Such as, AnnexinV-optimistic cells which are morphologically distinctive than wholesome cells, or CD14+ monocytes that happen to be greater and much more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it can be illuminating to determine how many doublets slip as a result of common singlet gates, and good to confirm the standing of sudden double-positive activities in my Examination."
Optimizing gating tactic. Even robust handbook singlet gating is mistake-vulnerable and remains a subjective conclusion place in Virtually all flow cytometry assays. Imaging can be employed to substantiate and alter gates to incorporate only one cells of fascination.
Our researchers will style and design panels in your case as many times you may need—cost-free, no merchandise obtain wanted.
Early indications of this contamination had in the beginning been dismissed as debris. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also permits you to use morphological capabilities to find attention-grabbing subpopulations that might not be obvious from flow cytometry info by yourself.
Most likely most of all, these occasions consist of cells of Obviously unique phenotypes which may lead to incorrect conclusions pertaining to double favourable situations (especially in scarce populations).
Seem waves enable precise mobile alignment, causing Outstanding information top quality and ~10X here more quickly sample flow fees.
We previously shown the power of imaging Motor vehicle-T/Ramos mobile interactions. Enable’s check out just the population of finest curiosity, the double beneficial functions, To find out more. We can now use prolonged morphological parameters (below, circularity and skewness of intensity) to further more examine the features of these populations and refine gating on these events, raising facts robustness.
Leverage the strength of Highly developed know-how and innovation to accelerate your discoveries. Believe in during the Attune Flow Cytometer family members to provide the dependability, pace, and accuracy that set your operate aside.
Versatile payment approach from Agilent Economical Answers allow you to obtain the vital instrumentation you need for instant project, or to expand your lab.
At the center of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technologies, which utilizes audio waves to meticulously align cells within the flow mobile. This progressive technique surpasses common hydrodynamic strategies, resulting in faster processing speeds, Remarkable info excellent, and enhanced sample throughput.
The Attune Xenith cytometer is actually a large-parameter spectral analyzer, offering both spectral unmixing and conventional flow abilities for comprehensive and adaptable cell analysis.
Agilent Price Assure assures you at the least a decade of instrument use through the day you purchase. Usually, we will credit score you with the residual price of the technique toward an upgraded design.
What seemed to be a homogeneous inhabitants is really a lot more assorted—an Perception that should be viewed as when interpreting final results.